(MRS / MRSI - Magnetic Resonance Spectroscopic Imaging) A method using the NMR phenomenon to identify the chemical state of various elements without destroying the sample. MRS therefore provides information about the chemical composition of the tissues and the changes in chemical composition, which may occur with disease processes.
Although MRS is primarily employed as a research tool and has yet to achieve widespread acceptance in routine clinical practice, there is a growing realization that a noninvasive technique, which monitors disease biochemistry can provide important new information for the clinician.
The underlying principle of MRS is that atomic nuclei are surrounded by a cloud of electrons, which very slightly shield the nucleus from any external magnetic field. As the structure of the electron cloud is specific to an individual molecule or compound, then the magnitude of this screening effect is also a characteristic of the chemical environment of individual nuclei.
In view of the fact that the resonant frequency is proportional to the magnetic field that it experiences, it follows that the resonant frequency will be determined not only by the external applied field, but also by the small field shift generated by the electron cloud. This shift in frequency is called the chemical shift (see also Chemical Shift). It should be noted that chemical shift is a very small effect, usually expressed in ppm of the main frequency. In order to resolve the different chemical species, it is therefore necessary to achieve very high levels of homogeneity of the main magnetic field B0. Spectra from humans usually require shimming the magnet to approximately one part in 100. High resolution spectra of liquid samples demand a homogeneity of about one part in 1000.
In addition to the effects of factors such as relaxation times that can affect the NMR signal, as seen in magnetic resonance imaging, effects such as J-modulation or the transfer of magnetization after selective excitation of particular spectral lines can affect the relative strengths of spectral lines.
In the context of human MRS, two nuclei are of particular interest - H-1 and P-31. (PMRS - Proton Magnetic Resonance Spectroscopy) PMRS is mainly employed in studies of the brain where prominent peaks arise from NAA, choline containing compounds, creatine and creatine phosphate, myo-inositol and, if present, lactate; phosphorus 31 MR spectroscopy detects compounds involved in energy metabolism (creatine phosphate, adenosine triphosphate and inorganic phosphate) and certain compounds related to membrane synthesis and degradation. The frequencies of certain lines may also be affected by factors such as the local pH. It is also possible to determine intracellular pH because the inorganic phosphate peak position is pH sensitive.
If the field is uniform over the volume of the sample, "similar" nuclei will contribute a particular frequency component to the detected response signal irrespective of their individual positions in the sample. Since nuclei of different elements resonate at different frequencies, each element in the sample contributes a different frequency component. A chemical analysis can then be conducted by analyzing the MR response signal into its frequency components.

See also Spectroscopy.

(PC) Phase contrast sequences are the basis of MRA techniques utilizing the change in the phase shifts of the flowing protons in the region of interest to create an image. Spins that are moving along the direction of a magnetic field gradient receive a phase shift proportional to their velocity.
In a phase contrast sequence two data sets with a different amount of flow sensitivity are acquired. This is usually accomplished by applying gradient pairs, which sequentially dephase and then rephase spins during the sequence. Both 2D and 3D acquisition techniques can be applied with phase contrast MRA.
The first data set is acquired with a flow compensated sequence, i. e. without flow sensitivity. The second data set is acquired with a flow sensitive sequence. The amount of flow sensitivity is controlled by the strength of the bipolar gradient pulse pair, which is incorporated into the sequence. Stationary tissue undergoes no effective phase change after the application of the two gradients. Caused by the different spatial localization of flowing blood to stationary tissue, it experiences a different size of the second bipolar gradient compared to the first. The result is a phase shift.
The raw data from the two data sets are subtracted. By comparing the phase of signals from each location in the two sequences the exact amount of motion induced phase change can be determined to have a map where pixel brightness is proportional to spatial velocity.
Phase contrast images represent the signal intensity of the velocity of spins at each point within the field of view. Regions that are stationary remain black while moving regions are represented as grey to white.
The phase shift is proportional to the spin's velocity, and this allows the quantitative assessment of flow velocities. The difference MRI signal has a maximum value for opposite directions. This velocity is typically referred to as venc, and depends on the pulse amplitude and distance between the gradient pulse pair. For velocities larger than venc the difference signal is decreased constantly until it gets zero. Therefore, in a phase contrast angiography it is important to correctly set the venc of the sequence to the maximum flow velocity which is expected during the measurement. High venc factors of the PC angiogram (more than 40 cm/sec) will selectively image the arteries (PCA - arteriography), whereas a venc factor of 20 cm/sec will perform the veins and sinuses (PCV or MRV - venography).

See also Flow Quantification, Contrast Enhanced MR Venography, Time of Flight Angiography, Time Resolved Imaging of Contrast Kinetics.

(STIR) Also called Short Tau (t) (inversion time) Inversion Recovery. STIR is a fat suppression technique with an inversion time t = T1 ln2 where the signal of fat is zero (T1 is the spin lattice relaxation time of the component that should be suppressed). To distinguish two tissue components with this technique, the T1 values must be different. Fluid Attenuation Inversion Recovery (FLAIR) is a similar technique to suppress water.
Inversion recovery doubles the distance spins will recover, allowing more time for T1 differences. A 180° preparation pulse inverts the net magnetization to the negative longitudinal magnetization prior to the 90° excitation pulse. This specialized application of the inversion recovery sequence set the inversion time (t) of the sequence at 0.69 times the T1 of fat. The T1 of fat at 1.5 Tesla is approximately 250 with a null point of 170 ms while at 0.5 Tesla its 215 with a 148 ms null point. At the moment of excitation, about 120 to 170 ms after the 180° inversion pulse (depending of the magnetic field) the magnetization of the fat signal has just risen to zero from its original, negative, value and no fat signal is available to be flipped into the transverse plane.
When deciding on the optimal T1 time, factors to be considered include not only the main field strength, but also the tissue to be suppressed and the anatomy. In comparison to a conventional spin echo where tissues with a short T1 are bright due to faster recovery, fat signal is reversed or darkened. Because body fluids have both a long T1 and a long T2, it is evident that STIR offers the possibility of extremely sensitive detection of body fluid. This is of course, only true for stationary fluid such as edema, as the MRI signal of flowing fluids is governed by other factors.

See also Fat Suppression and Inversion Recovery Sequence.

A constant for any given nucleus that relates the nuclear MR frequency and the strength of the external magnetic field.
Definition: The ratio of the magnetic moment (field strength = T) to the angular momentum (frequency = ν) of a particle.
The gyromagnetic effect happens if a magnetic substance is subjected to a magnetic field. Upon a change in direction of the magnetic field, the magnetization of the substance must change. In order for this to happen, the atoms must change their angular momentum. Since there are no external torques acting on the system, the total angular momentum must remain constant. This mass rotation may be measured. The gyromagnetic ratio is different for each nucleus of different atoms. The value of the gyromagnetic ratio for hydrogen (1H) is 4,258 (Hz/G) (42.58 MHz/T).

A feedback control used to maintain the static magnetic field at a constant strength, usually by monitoring the resonance frequency of a reference sample or line in the spectrum.